Cysteine-free glutathione-S-tranferase as a tool for thiol-specific labeling of proteins.
نویسندگان
چکیده
منابع مشابه
S-cysteinylation is a general mechanism for thiol protection of Bacillus subtilis proteins after oxidative stress.
S-Thiolation is crucial for protection and regulation of thiol-containing proteins during oxidative stress and is frequently achieved by the formation of mixed disulfides with glutathione. However, many Gram-positive bacteria including Bacillus subtilis lack the low molecular weight (LMW) thiol glutathione. Here we provide evidence that S-thiolation by the LMW thiol cysteine represents a genera...
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عنوان ژورنال:
- BioTechniques
دوره 40 2 شماره
صفحات -
تاریخ انتشار 2006